In Experiment 1B you are hoping to isolate mutations in the HIS1 gene of yeast using CRISPR/Cas9.

In Experiment 1B you are hoping to isolate mutations in the HIS1 gene of yeast using CRISPR/Cas9. In this experiment, how will you determine that you obtained mutations specifically in the HIS1 gene, and not in some other gene required for histidine biosynthesis?